Lanthanide nanoparticles for high sensitivity multiparameter single cell analysis

Multicolor tuning of lanthanide-doped nanoparticles by single wavelength excitation.

Lanthanide-doped nanoparticles exhibit unique luminescent properties, including large Stokes shift, sharp emission bandwidth, high resistance to optical blinking, and photobleaching, as well as the unique ability to convert long-wavelength stimulation into short-wavelength emission. These attributes are particularly needed for developing luminescent labels as alternatives to organic fluorophore...

Lanthanide - doped upconversion nanoparticles

Lanthanide-Doped Nanoparticles for Diagnostic Sensing

Lanthanide-doped nanoparticles exhibit unique optical properties, such as a long luminescence lifetime (up to several milliseconds), sharp emission peaks, and upconversion luminescence over the range of wavelengths from near-infrared to visible. Exploiting these optical properties, lanthanide-doped nanoparticles have been widely utilized for cellular and small animal imaging with the absence of...

Causal protein-signaling networks derived from multiparameter single-cell data.

Machine learning was applied for the automated derivation of causal influences in cellular signaling networks. This derivation relied on the simultaneous measurement of multiple phosphorylated protein and phospholipid components in thousands of individual primary human immune system cells. Perturbing these cells with molecular interventions drove the ordering of connections between pathway comp...

Karen Sachs , Multiparameter Single - Cell Data Causal Protein - Signaling Networks

www.sciencemag.org (this information is current as of March 30, 2008 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/sci;309/5738/1187a A correction has been published for this article at: http://www.sciencemag.org/cgi/content/full/308/5721/523 version of this article at: including high-resolution figures, can be found in the...